Sucrose Uptake by Developing Soybean Cotyledons
نویسندگان
چکیده
منابع مشابه
Sucrose uptake by developing soybean cotyledons.
Sucrose uptake by excised developing soybean cotyledons shows a biphasic dependence on sucrose concentration. At concentrations less than about 50 millimolar external sucrose, uptake can be described as a carrier-mediated process, with a K(m) of 8 millimolar. At higher external sucrose concentrations, a linear dependence becomes apparent, which suggests the participation of a nonsaturable compo...
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Addition of sucrose to a solution bathing an excised developing soybean cotyledon causes a transient depolarization of the membrane potential, as measured using standard electrophysiological techniques. The magnitude of the depolarization is dependent on the concentration of both sucrose and protons in a manner which suggests carrier mediation; this process has an apparent K(m) for sucrose of a...
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The accumulation of tetraphenylphosphonium (TPP(+)), 5,5'-dimethyl-oxazolidine-2,4-dione (DMO), and a micro pH electrode were used to measure membrane potential, intracellular and extracellular pH, respectively, upon the addition of exogenous sucrose to soybean cotyledon protoplasts. Addition of sucrose caused a specific and transient (a) depolarization of the membrane potential (measured by TP...
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Tracerkinetic experiments were performed using L-[guanidino14C]arginine, L-[U-14C]arginine, L-[ureido-14C]citrulline, and L-[114C]omithine to investigate arginine utilization in developing cotyledons of Glycine max (L.) Merrill. Excised cotyledons were injected with carrier-free 14C compounds and incubated in sealed vials containing a CO2 trap. The free and protein amino acids were analyzed usi...
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The incorporation of phospholipids specifically labeled with glycerol-2(3)H and acyl-(14)C by whole cell tissues of developing soybean cotyledons (Glycine max L.) reveals that phosphatidylinositol, phosphatidylcholine, phosphatidylethanolamine, N-acylphosphatidylethanolamine, and phosphatidic acid can be metabolized to diglyceride. The diglyceride formed may be recylced into phospholipid or acy...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1981
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.68.3.693